DNA

Part:BBa_K4769103:Design

Designed by: Kieran Abbott   Group: iGEM23_Cambridge   (2023-10-10)


spoIIE knockout 5’ integration sequence for B. subtilis 3610


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

B. subtilis 3610 genome

Primers for cloning: FW: gctgataattatgtcaaagacattaacgac (+ desired overhangs) RV: cattcctctcatctcccacctg (+ desired overhangs)


References